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OCR: acted The separation step eliminates all nor the components. According to the label us neasured activity of the bound fraction is directl (RIA, FIA) or a further reagent is added development of colour or light (EIA, e chemoluminescent assay). By plotting concentration of the standards against be measured response, a standard curve c response constructed. In the competitive assay, t of the is inversely related to the concentration antigen in the sample (Fig. 22.12).